Science & Technology
Scientists identify a plant molecule that sops up iron-rich heme
The peptide is used by legumes to control nitrogen-fixing bacteria; it may also offer leads for treating patients with too much heme in their blood

Written by Anne Trafton, MIT News Office
Symbiotic relationships between legumes and the bacteria that grow in their roots are critical for plant survival. Without those bacteria, the plants would have no source of nitrogen, an element that is essential for building proteins and other biomolecules, and they would be dependent on nitrogen fertilizer in the soil.
To establish that symbiosis, some legume plants produce hundreds of peptides that help bacteria live within structures known as nodules within their roots. A new study from MIT reveals that one of these peptides has an unexpected function: It sops up all available heme, an iron-containing molecule. This sends the bacteria into an iron-starvation mode that ramps up their production of ammonia, the form of nitrogen that is usable for plants.
“This is the first of the 700 peptides in this system for which a really detailed molecular mechanism has been worked out,” says Graham Walker, the American Cancer Society Research Professor of Biology at MIT, a Howard Hughes Medical Institute Professor, and the senior author of the study.
This heme-sequestering peptide could have beneficial uses in treating a variety of human diseases, the researchers say. Removing free heme from the blood could help to treat diseases caused by bacteria or parasites that need heme to survive, such as P. gingivalis (periodontal disease) or toxoplasmosis, or diseases such as sickle cell disease or sepsis that release too much heme into the bloodstream.
“This study demonstrates that basic research in plant-microbe interactions also has potential to be translated to therapeutic applications,” says Siva Sankari, an MIT research scientist and the lead author of the study, which appears today in Nature Microbiology.
Other authors of the paper include Vignesh Babu, an MIT research scientist; Kevin Bian and Mary Andorfer, both MIT postdocs; Areej Alhhazmi, a former KACST-MIT Ibn Khaldun Fellowship for Saudi Arabian Women scholar; Kwan Yoon and Dante Avalos, MIT graduate students; Tyler Smith, an MIT instructor in biology; Catherine Drennan, an MIT professor of chemistry and biology and a Howard Hughes Medical Institute investigator; Michael Yaffe, a David H. Koch Professor of Science and a member of MIT’s Koch Institute for Integrative Cancer Research; and Sebastian Lourido, the Latham Family Career Development Professor of Biology at MIT and a member of the Whitehead Institute for Biomedical Research.
Iron control
For nearly 40 years, Walker’s lab has been studying the symbiosis between legumes and rhizobia, a type of nitrogen-fixing bacteria. These bacteria convert nitrogen gas to ammonia, a critical step of the Earth’s nitrogen cycle that makes the element available to plants (and to animals that eat the plants).
Most of Walker’s work has focused on a clover-like plant called Medicago truncatula. Nitrogen-fixing bacteria elicit the formation of nodules on the roots of these plants and eventually end up inside the plant cells, where they convert to their symbiotic form called bacteroids.
Several years ago, plant biologists discovered that Medicago truncatula produces about 700 peptides that contribute to the formation of these bacteroids. These peptides are generated in waves that help the bacteria make the transition from living freely to becoming embedded into plant cells where they act as nitrogen-fixing machines.
Walker and his students picked one of these peptides, known as NCR247, to dig into more deeply. Initial studies revealed that when nitrogen-fixing bacteria were exposed to this peptide, 15 percent of their genes were affected. Many of the genes that became more active were involved in importing iron.
The researchers then found that when they fused NCR247 to a larger protein, the hybrid protein was unexpectedly reddish in color. This serendipitous observation led to the discovery that NCR247 binds heme, an organic ring-shaped iron-containing molecule that is an important component of hemoglobin, the protein that red blood cells use to carry oxygen.
Further studies revealed that when NCR247 is released into bacterial cells, it sequesters most of the heme in the cell, sending the cells into an iron-starvation mode that triggers them to begin importing more iron from the external environment.
“Usually bacteria fine-tune their iron metabolism, and they don’t take up more iron when there is already enough,” Sankari says. “What’s cool about this peptide is that it overrides that mechanism and indirectly regulates the iron content of the bacteria.”
Nitrogenase, the main enzyme that bacteria use to fix nitrogen, requires 24 to 32 atoms of iron per enzyme molecule, so the influx of extra iron likely helps those enzymes to become more active, the researchers say. This influx is timed to coincide with nitrogen fixation, they found.
“These peptides are produced in a wave in the nodules, and the production of this particular peptide is higher when the bacteria are preparing to fix nitrogen. If this peptide was secreted throughout the whole process, then the cell would have too much iron all the time, which is bad for the cell,” Sankari says.
Without the NCR247 peptide, Medicago truncatula and rhizobium cannot form an effective nitrogen-fixing symbiosis, the researchers showed.
“Many possible directions”
The peptide that the researchers studied in this work may have potential therapeutic uses. When heme is incorporated into hemoglobin, it performs a critical function in the body, but when it’s loose in the bloodstream, it can kill cells and promote inflammation. Free heme can accumulate in stored blood, so having a way to filter out the heme before the blood is transfused into a patient could be potentially useful.
A variety of human diseases lead to free heme circulating in the bloodstream, including sickle cell anemia, sepsis, and malaria. Additionally, some infectious parasites and bacteria depend on heme for their survival but cannot produce it, so they scavenge it from their environment. Treating such infections with a protein that takes up all available heme could help prevent the parasitic or bacterial cells from being able to grow and reproduce.
In this study, Lourido and members of his lab showed that treating the parasite Toxoplasma gondii with NCR427 prevented the parasite from forming plaques on human cells.
The researchers are now pursuing collaborations with other labs at MIT to explore some of these potential applications, with funding from a Professor Amar G. Bose Research Grant.
“There are many possible directions, but they’re all at a very early stage,” Walker says. “The number of potential clinical applications is very broad. You can place more than one bet in this game, which is an intriguing thing.”
Currently, the human protein hemopexin, which also binds to heme, is being explored as a possible treatment for sickle cell anemia. The NCR247 peptide could provide an easier to deploy alternative, the researchers say, because it is much smaller and could be easier to manufacture and deliver into the body.
The research was funded in part by the MIT Center for Environmental Health Sciences, the National Science Foundation, and the National Institutes of Health.
Science & Technology
Speedy robo-gripper reflexively organizes cluttered spaces
Rather than start from scratch after a failed attempt, the pick-and-place robot adapts in the moment to get a better hold

Written by Jennifer Chu, MIT News Office
Images/video: https://news.mit.edu/2023/speedy-robo-gripper-reflexively-organizes-spaces-0427
When manipulating an arcade claw, a player can plan all she wants. But once she presses the joystick button, it’s a game of wait-and-see. If the claw misses its target, she’ll have to start from scratch for another chance at a prize.
The slow and deliberate approach of the arcade claw is similar to state-of-the-art pick-and-place robots, which use high-level planners to process visual images and plan out a series of moves to grab for an object. If a gripper misses its mark, it’s back to the starting point, where the controller must map out a new plan.

Credits:Image: Jodi Hilton
Looking to give robots a more nimble, human-like touch, MIT engineers have now developed a gripper that grasps by reflex. Rather than start from scratch after a failed attempt, the team’s robot adapts in the moment to reflexively roll, palm, or pinch an object to get a better hold. It’s able to carry out these “last centimeter” adjustments (a riff on the “last mile” delivery problem) without engaging a higher-level planner, much like how a person might fumble in the dark for a bedside glass without much conscious thought.
The new design is the first to incorporate reflexes into a robotic planning architecture. For now, the system is a proof of concept and provides a general organizational structure for embedding reflexes into a robotic system. Going forward, the researchers plan to program more complex reflexes to enable nimble, adaptable machines that can work with and among humans in ever-changing settings.
“In environments where people live and work, there’s always going to be uncertainty,” says Andrew SaLoutos, a graduate student in MIT’s Department of Mechanical Engineering. “Someone could put something new on a desk or move something in the break room or add an extra dish to the sink. We’re hoping a robot with reflexes could adapt and work with this kind of uncertainty.”
SaLoutos and his colleagues will present a paper on their design in May at the IEEE International Conference on Robotics and Automation (ICRA). His MIT co-authors include postdoc Hongmin Kim, graduate student Elijah Stanger-Jones, Menglong Guo SM ’22, and professor of mechanical engineering Sangbae Kim, the director of the Biomimetic Robotics Laboratory at MIT.
High and low
Many modern robotic grippers are designed for relatively slow and precise tasks, such as repetitively fitting together the same parts on a a factory assembly line. These systems depend on visual data from onboard cameras; processing that data limits a robot’s reaction time, particularly if it needs to recover from a failed grasp.
“There’s no way to short-circuit out and say, oh shoot, I have to do something now and react quickly,” SaLoutos says. “Their only recourse is just to start again. And that takes a lot of time computationally.”
In their new work, Kim’s team built a more reflexive and reactive platform, using fast, responsive actuators that they originally developed for the group’s mini cheetah — a nimble, four-legged robot designed to run, leap, and quickly adapt its gait to various types of terrain.
The team’s design includes a high-speed arm and two lightweight, multijointed fingers. In addition to a camera mounted to the base of the arm, the team incorporated custom high-bandwidth sensors at the fingertips that instantly record the force and location of any contact as well as the proximity of the finger to surrounding objects more than 200 times per second.
The researchers designed the robotic system such that a high-level planner initially processes visual data of a scene, marking an object’s current location where the gripper should pick the object up, and the location where the robot should place it down. Then, the planner sets a path for the arm to reach out and grasp the object. At this point, the reflexive controller takes over.
If the gripper fails to grab hold of the object, rather than back out and start again as most grippers do, the team wrote an algorithm that instructs the robot to quickly act out any of three grasp maneuvers, which they call “reflexes,” in response to real-time measurements at the fingertips. The three reflexes kick in within the last centimeter of the robot approaching an object and enable the fingers to grab, pinch, or drag an object until it has a better hold.
They programmed the reflexes to be carried out without having to involve the high-level planner. Instead, the reflexes are organized at a lower decision-making level, so that they can respond as if by instinct, rather than having to carefully evaluate the situation to plan an optimal fix.
“It’s like how, instead of having the CEO micromanage and plan every single thing in your company, you build a trust system and delegate some tasks to lower-level divisions,” Kim says. “It may not be optimal, but it helps the company react much more quickly. In many cases, waiting for the optimal solution makes the situation much worse or irrecoverable.”
Cleaning via reflex
The team demonstrated the gripper’s reflexes by clearing a cluttered shelf. They set a variety of household objects on a shelf, including a bowl, a cup, a can, an apple, and a bag of coffee grounds. They showed that the robot was able to quickly adapt its grasp to each object’s particular shape and, in the case of the coffee grounds, squishiness. Out of 117 attempts, the gripper quickly and successfully picked and placed objects more than 90 percent of the time, without having to back out and start over after a failed grasp.
A second experiment showed how the robot could also react in the moment. When researchers shifted a cup’s position, the gripper, despite having no visual update of the new location, was able to readjust and essentially feel around until it sensed the cup in its grasp. Compared to a baseline grasping controller, the gripper’s reflexes increased the area of successful grasps by over 55 percent.
Now, the engineers are working to include more complex reflexes and grasp maneuvers in the system, with a view toward building a general pick-and-place robot capable of adapting to cluttered and constantly changing spaces.
“Picking up a cup from a clean table — that specific problem in robotics was solved 30 years ago,” Kim notes. “But a more general approach, like picking up toys in a toybox, or even a book from a library shelf, has not been solved. Now with reflexes, we think we can one day pick and place in every possible way, so that a robot could potentially clean up the house.”
This research was supported, in part, by Advanced Robotics Lab of LG Electronics and the Toyota Research Institute.
Science & Technology
Researchers 3D print a miniature vacuum pump
The device would be a key component of a portable mass spectrometer that could help monitor pollutants or perform medical diagnoses in remote parts of the world

Written by Adam Zewe, MIT News Office
Mass spectrometers are extremely precise chemical analyzers that have many applications, from evaluating the safety of drinking water to detecting toxins in a patient’s blood. But building an inexpensive, portable mass spectrometer that could be deployed in remote locations remains a challenge, partly due to the difficulty of miniaturizing the vacuum pump it needs to operate.
MIT researchers utilized additive manufacturing to take a major step toward solving this problem. They 3D printed a miniature version of a type of vacuum pump, known as a peristaltic pump, that is about the size of a human fist.
Their pump can create and maintain a vacuum that has an order of magnitude lower pressure than another type of commonly used pump. The unique design, which can be printed in one pass on a multimaterial 3D printer, prevents fluid or gas from leaking while minimizing heat from friction during the pumping process. This increases the lifetime of the device.

Credits:Image: Courtesy of the researchers
This pump could be incorporated into a portable mass spectrometer used to monitor soil contamination in isolated parts of the world, for instance. The device could also be ideal for use in geological survey equipment bound for Mars, since it would be cheaper to launch the lightweight pump into space.
“We are talking about very inexpensive hardware that is also very capable. With mass spectrometers, the 500-pound gorilla in the room has always been the issue of pumps. What we have shown here is groundbreaking, but it is only possible because it is 3D-printed. If we wanted to do this the standard way, we wouldn’t have been anywhere close,” says Luis Fernando Velásquez-García, a principal scientist in MIT’s Microsystems Technology Laboratories (MTL) and senior author of a paper describing the new pump.
Velásquez-García is joined on the paper by lead author Han-Joo Lee, a former MIT postdoc; and Jorge Cañada Pérez-Sala, an electrical engineering and computer science graduate student. The paper appears today in Additive Manufacturing.
Pump problems
As a sample is pumped through a mass spectrometer, it is hit with an electric charge to turn its atoms into ions. An electromagnetic field manipulates these ions in a vacuum so their masses can be determined. This information can be used to identify the molecules in the sample. Maintaining the vacuum is key because if the ions collide with gas molecules from the air, their dynamics will change.
Peristaltic pumps are commonly used to move fluids or gases that would contaminate the pump’s components, such as reactive chemicals. The substance is entirely contained within a flexible tube that is looped around a set of rollers. The rollers squeeze the tube against its housing as they rotate. The pinched parts of the tube expand in the wake of the rollers, creating a vacuum that draws the liquid or gas through the tube.
While the pumps do create a vacuum, design problems have limited their use in mass spectrometers. The tube material redistributes when force is applied by the rollers, leading to gaps that cause leaks. This problem can be overcome by operating the pump rapidly, forcing the fluid through faster than it can leak out. But this causes excessive heat that damages the pump, and the gaps remain. To fully seal the tube and create the vacuum needed for a mass spectrometer, the mechanism must exert additional force to squeeze the bulged areas, causing more damage, explains Velásquez-García.
An additive solution
He and his team rethought the peristaltic pump design from the bottom up, looking for ways they could use additive manufacturing to make improvements. First, by using a multimaterial 3D printer, they were able to make the flexible tube out of a special type of hyperelastic material that can withstand a huge amount of deformation.
Then, through an iterative design process, they determined that adding notches to the walls of the tube would reduce the stress on the material when squeezed. With notches, the tube material does not need to redistribute to counteract the force from the rollers.
The precision afforded by 3D printing enabled the researchers to produce the exact notch size needed to eliminate the gaps. They were also able to vary the tube’s thickness so the walls are stronger in areas where connectors attach, further reducing stress on the material.
Using a multimaterial 3D printer, they printed the entire tube in one pass, which is important since postassembly can introduce defects that can cause leaks. To do this, they had to find a way to print the narrow, flexible tube vertically while preventing it from wobbling during the process. In the end, they created a lightweight structure that stabilizes the tube during printing but can be easily peeled off later without damaging the device.
“One of the key advantages of using 3D printing is that it allows us to aggressively prototype. If you do this work in a clean room, where a lot of these miniaturized pumps are made, it takes a lot of time. If you want to make a change, you have to start the entire process over. In this case, we can print our pump in a matter of hours, and every time it can be a new design,” Velásquez-García says.
Portable, yet performant
When they tested their final design, the researchers found that it was able to create a vacuum that had an order of magnitude lower pressure than state-of-the-art diaphragm pumps. Lower pressure yields a higher-quality vacuum. To reach that same pressure with standard pumps, one would need to connect three in a series, Velásquez-García says.
The pump reached a maximum temperature of 50 degrees Celsius, half that of state-of-the-art pumps used in other studies, and only required half as much force to fully seal the tube.
In the future, the researchers plan to explore ways to further reduce the maximum temperature, which would enable the tube to actuate faster, creating a better vacuum and increasing the flow rate. They are also working to 3D print an entire miniaturized mass spectrometer. As they develop that device, they will continue fine-tuning the specifications of the peristaltic pump.
“Some people think that when you 3D print something there must be some kind of tradeoff. But here our group has shown that is not the case. It really is a new paradigm. Additive manufacturing is not going to solve all the problems of the world, but it is a solution that has real legs,” Velásquez-García says.
This work was supported, in part, by the Empiriko Corporation.
Science & Technology
Miniscule device could help preserve the battery life of tiny sensors
Researchers demonstrate a low-power “wake-up” receiver one-tenth the size of other devices

Written by Adam Zewe, MIT News Office
Scientists are striving to develop ever-smaller internet-of-things devices, like sensors tinier than a fingertip that could make nearly any object trackable. These diminutive sensors have miniscule batteries which are often nearly impossible to replace, so engineers incorporate wake-up receivers that keep devices in low-power “sleep” mode when not in use, preserving battery life.
Researchers at MIT have developed a new wake-up receiver that is less than one-tenth the size of previous devices and consumes only a few microwatts of power. Their receiver also incorporates a low-power, built-in authentication system, which protects the device from a certain type of attack that could quickly drain its battery.
Many common types of wake-up receivers are built on the centimeter scale since their antennas must be proportional to the size of the radio waves they use to communicate. Instead, the MIT team built a receiver that utilizes terahertz waves, which are about one-tenth the length of radio waves. Their chip is barely more than 1 square millimeter in size.
They used their wake-up receiver to demonstrate effective, wireless communication with a signal source that was several meters away, showcasing a range that would enable their chip to be used in miniaturized sensors.
For instance, the wake-up receiver could be incorporated into microrobots that monitor environmental changes in areas that are either too small or hazardous for other robots to reach. Also, since the device uses terahertz waves, it could be utilized in emerging applications, such as field-deployable radio networks that work as swarms to collect localized data.
“By using terahertz frequencies, we can make an antenna that is only a few hundred micrometers on each side, which is a very small size. This means we can integrate these antennas to the chip, creating a fully integrated solution. Ultimately, this enabled us to build a very small wake-up receiver that could be attached to tiny sensors or radios,” says Eunseok Lee, an electrical engineering and computer science (EECS) graduate student and lead author of a paper on the wake-up receiver.
Lee wrote the paper with his co-advisors and senior authors Anantha Chandrakasan, dean of the MIT School of Engineering and the Vannevar Bush Professor of Electrical Engineering and Computer Science, who leads the Energy-Efficient Circuits and Systems Group, and Ruonan Han, an associate professor in EECS, who leads the Terahertz Integrated Electronics Group in the Research Laboratory of Electronics; as well as others at MIT, the Indian Institute of Science, and Boston University. The research is being presented at the IEEE Custom Integrated Circuits Conference.
Scaling down the receiver
Terahertz waves, found on the electromagnetic spectrum between microwaves and infrared light, have very high frequencies and travel much faster than radio waves. Sometimes called “pencil beams,” terahertz waves travel in a more direct path than other signals, which makes them more secure, Lee explains.
However, the waves have such high frequencies that terahertz receivers often multiply the terahertz signal by another signal to alter the frequency, a process known as frequency mixing modulation. Terahertz mixing consumes a great deal of power.
Instead, Lee and his collaborators developed a zero-power-consumption detector that can detect terahertz waves without the need for frequency mixing. The detector uses a pair of tiny transistors as antennas, which consume very little power.
Even with both antennas on the chip, their wake-up receiver was only 1.54 square millimeters in size and consumed less than 3 microwatts of power. This dual-antenna setup maximizes performance and makes it easier to read signals.
Once received, their chip amplifies a terahertz signal and then converts analog data into a digital signal for processing. This digital signal carries a token, which is a string of bits (0s and 1s). If the token corresponds to the wake-up receiver’s token, it will activate the device.
Ramping up security
In most wake-up receivers, the same token is reused multiple times, so an eavesdropping attacker could figure out what it is. Then the hacker could send a signal that would activate the device over and over again, using what is called a denial-of-sleep attack.
“With a wake-up receiver, the lifetime of a device could be improved from one day to one month, for instance, but an attacker could use a denial-of-sleep attack to drain that entire battery life in even less than a day. That is why we put authentication into our wake-up receiver,” he explains.
They added an authentication block that utilizes an algorithm to randomize the device’s token each time, using a key that is shared with trusted senders. This key acts like a password — if a sender knows the password, they can send a signal with the right token. The researchers do this using a technique known as lightweight cryptography, which ensures the entire authentication process only consumes a few extra nanowatts of power.
They tested their device by sending terahertz signals to the wake-up receiver as they increased the distance between the chip and the terahertz source. In this way, they tested the sensitivity of their receiver — the minimum signal power needed for the device to successfully detect a signal. Signals that travel farther have less power.
“We achieved 5- to 10-meter longer distance demonstrations than others, using a device with a very small size and microwatt level power consumption,” Lee says.
But to be most effective, terahertz waves need to hit the detector dead-on. If the chip is at an angle, some of the signal will be lost. So, the researchers paired their device with a terahertz beam-steerable array, recently developed by the Han group, to precisely direct the terahertz waves. Using this technique, communication could be sent to multiple chips with minimal signal loss.
In the future, Lee and his collaborators want to tackle this problem of signal degradation. If they can find a way to maintain signal strength when receiver chips move or tilt slightly, they could increase the performance of these devices. They also want to demonstrate their wake-up receiver in very small sensors and fine-tune the technology for use in real-world devices.
“We have developed a rich technology portfolio for future millimeter-sized sensing, tagging, and authentication platforms, including terahertz backscattering, energy harvesting, and electrical beam steering and focusing. Now, this portfolio is more complete with Eunseok’s first-ever terahertz wake-up receiver, which is critical to save the extremely limited energy available on those mini platforms,” Han says.
Additional co-authors include Muhammad Ibrahim Wasiq Khan PhD ’22; Xibi Chen, an EECS graduate student; Ustav Banerjee PhD ’21, an assistant professor at the Indian Institute of Science; Nathan Monroe PhD ’22; and Rabia Tugce Yazicigil, an assistant professor of electrical and computer engineering at Boston University.
-
Business & Economy1 year ago
NSE Academy Limited collaborates with HDFC Mutual Fund for financial awareness program
-
Edu News2 months ago
Innovative Ideas and Breakthroughs from NMIMS MPSTME Civil Engineering
-
Science & Technology3 months ago
3D-printed revolving devices can sense how they are moving
-
Business & Economy10 months ago
Using artificial intelligence to control digital manufacturing
-
Edu News1 year ago
Technique protects privacy when making online recommendations
-
Edu News12 months ago
Astronomers discover a multiplanet system nearby
-
Edu News12 months ago
Stronger security for smart devices
-
Edu News1 year ago
Search reveals eight new sources of black hole echoes